Protein Thiocarboxylate-Dependent Methionine Biosynthesis inWolinella succinogenes

Biosynthesis of methionine in liver.

Conversion of 1 carbon precursors to methionine methyl groups in a cell-free preparation was first demonstrated by Berg in this laboratory (2). Berg inactivated a pigeon liver extract by Dowex I-Cltreatment and dialysis and found that it could be activated by a boiled extract of pigeon liver. Methionine methyl formation in dialyzed liver estracts has been activated recently by mixtures of known...

Acetylhomoserine and methionine biosynthesis in Neurospora.

Cobalamin-dependent methionine synthase.

Cobalamin-dependent methionine synthase catalyzes the transfer of a methyl group from N5-methyltetrahydrofolate to homocysteine, producing tetrahydrofolate and methionine. Insufficient availability of cobalamin, or inhibition of methionine synthase by exposure to nitrous oxide, leads to diminished activity of this enzyme. In humans, severe inhibition of methionine synthase results in the develo...

Divergent evolution of an atypical S-adenosyl-l-methionine-dependent monooxygenase involved in anthracycline biosynthesis.

Bacterial secondary metabolic pathways are responsible for the biosynthesis of thousands of bioactive natural products. Many enzymes residing in these pathways have evolved to catalyze unusual chemical transformations, which is facilitated by an evolutionary pressure promoting chemical diversity. Such divergent enzyme evolution has been observed in S-adenosyl-L-methionine (SAM)-dependent methyl...

Comprehensive Profiling of Amino Acid Response Uncovers Unique Methionine-Deprived Response Dependent on Intact Creatine Biosynthesis

Besides being building blocks for protein synthesis, amino acids serve a wide variety of cellular functions, including acting as metabolic intermediates for ATP generation and for redox homeostasis. Upon amino acid deprivation, free uncharged tRNAs trigger GCN2-ATF4 to mediate the well-characterized transcriptional amino acid response (AAR). However, it is not clear whether the deprivation of d...